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CounterACT Center Publications

A longitudinal MRI and TSPO PET-based investigation of brain region-specific neuroprotection by diazepam versus midazolam following organophosphate-induced seizures.
Hobson BA, Rowland DR, Dou Y, Saito N, Harmany ZT, Bruun DA, Harvey DJ, Chaudhari A, Garbow JR, Lein PJ.
Neuropharmacology. 2024 Jun 15:251:109918. PMID: 38527652 PMCID - in process

Quantitative T2 mapping-based longitudinal assessment of brain injury and therapeutic rescue in the rat following acute organophosphate intoxication.
Almeida AJD, Hobson BA, Saito N, Bruun DA, Porter VA, Harvey DJ, Garbow JR, Chaudhari AJ, Lein PJ.
Neuropharmacology. 2024 May 15;249:109895. PMID: 38437913 PMCID - in process

Fully automated whole brain segmentation from rat MRI scans with a convolutional neural network.
Porter VA, Hobson BA, Foster B, Lein PJ, Chaudhari AJ.J
Neurosci Methods. 2024 May:405:110078. PMCID: PMC11000587 (available on 2025-02-01)

Cardiovascular responses of adult male Sprague-Dawley rats following acute organophosphate intoxication and post-exposure treatment with midazolam with or without allopregnanolone.
Pan S, Bruun DA, Lein PJ, Chen CY.
Arch Toxicol. 2024 Apr;98(4):1177-1189. PMCID: PMC10944447

Acute intoxication with diisopropylfluorophosphate promotes cellular senescence in the adult male rat brain
Tsai YH, González EA, Grodzki AC, Bruun DA, Saito NH, Harvey DJ, Lein PJ. 
Front Toxicol. 2024 Apr; 6: 1360359. doi: 10.3389/ftox.2024.1360359. PMCID: PMC11091247

The α4 nicotinic acetylcholine receptor Is necessary for the initiation of organophosphate-induced neuronal hyperexcitability
Andrew PM, Feng W, Calsbeek JJ, Antrobus SP, Cherednychenko GA, MacMahon JA, Bernardino PN, Liu X, Harvey DJ, Lein PJ, Pessah IN. 
Toxics. 2024 Mar 31;12(4):263  PMCID: PMC11054284

Inhibition of soluble epoxide hydrolase reduces inflammation and myocardial injury in arrhythmogenic cardiomyopathy. 
Panigrahy D, Kelly AG, Wang W, Yang J, Hwang SH, Gillespie M, Howard I, Bueno-Beti C, Asimaki A, Penna V, Lavine K, Edin ML, Zeldin DC, Hammock BD, Saffitz JE. 
bioRxiv [Preprint]. 2024 Feb 19:2024.02.17.580812. PMCID: PMC10925075

Evidence implicating blood-brain barrier impairment in the pathogenesis of acquired epilepsy following acute organophosphate intoxication.
Bernardino PN, Luo AS, Andrew PM, Unkel CM, Gonzalez MI, Gelli A, Lein PJ.
J Pharmacol Exp Ther. 2024 Jan 17;388(2):301-312. PMCID: PMC10801776


Soluble epoxide hydrolase-targeting PROTAC activates AMPK and inhibits endoplasmic reticulum stress. 
Peyman M, Barroso E, Turcu AL, Estrany Jr, F; Smith D, Jurado-Aguilar J, Rada P, Morisseau C, Hammock BD, Valverde ÁM, Palomer X, Galdeano C, Vázquez S, Vázquez-Carrera M. 
Biomed Pharmacother 2023 Dec:168:115667. PMCID: PMC10786198 (available on 2024-12-01)

Determinants of meal-induced changes in circulating FFA epoxides, diols, and diol-to-epoxide ratios as indices of soluble epoxide hydrolase activity. 
Oh YT, Yang J, Stefanovski D, Hammock B, Youn JH. 
Int J Mol Sci. 2023 Dec 11;24(24):17351. PMCID: PMC10743433.

Time- and region-dependent blood-brain barrier impairment in a rat model of organophosphate-induced status epilepticus.
Bernardino PN, Hobson BA, Huddleston SL, Andrew PM, MacMahon JA, Saito NH, Porter VA, Bruun DA, Harvey DJ, Garbow JR, Gelli A, Chaudhari AJ, Lein PJ. 
Neurobiol Dis. 2023 Oct 15;187:106316. PMCID: PMC11000668.

The rehabilitation potential of neurostimulation for mild traumatic brain injury in animal and human studies. 
McNerney MW, Gurkoff GG, Beard C, Berryhill ME. 
Brain Sci. 2023 Sep 30;13(10):1402. PMCID: PMC10605899

Quantification of soluble epoxide hydrolase inhibitors in experimental and clinical samples using the nanobody-based ELISA. 
Yang H, Qi M, He Q, Hwang SH, Yang J, McCoy M, Morisseau C, Zhao S, Hammock BD. 
J Pharm Anal. 2023 Sep; 13(9):1013–1023. PMCID: PMC10568103 

Tolerability and pharmacokinetics of intravenous allopregnanolone with and without midazolam pretreatment in two healthy dogs.
Bruun DA, Ma B, Chen YJ, Wu CY, Aleman M, Zolkowska D, Smiley-Jewell SM, Rogawski MA, Lein PJ.
Epilepsia Open. 2023 Jun;8(2):666-672. PMCID: PMC10235566

Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) causes seizure activity in larval zebrafish via antagonism of γ-aminobutyric acid type A receptor α1β2γ2.
Mundy PC, Werner A, Singh L, Singh V, Mendieta R, Patullo CE, Wulff H, Lein PJ.
Arch Toxicol. 2023 May;97(5):1355-1365. PMCID: PMC10110628

Cmpd10357 to treat B-cell acute lymphoblastic leukemia
Lee AQ, Konishi H, Helmke E, Ijiri M, Lerot JMA, Hicks E, Chien JR, Gorin FA, Satake N
Experimental Hematology. 2023 Mar-Apr;119-120:8-13.e1. PMCID: PMC10033359

Novel image analysis tool for rapid screening of cell morphology in preclinical animal models of disease.
Guignet M, Schmuck M, Harvey DJ, Nguyen D, Bruun D, Echeverri A, Gurkoff G, Lein PJ. Heliyon. 2023 Feb 8;9(2):e13449. PMCID: PMC9975095.

--2022--      Note: Current CounterACT Center began 1 September 2022. Previous Center publications are listed below.

The seizure-inducing plastic explosive RDX inhibits the α1β2γ2 GABA A receptor
Pressly B, Lee RD, Singh V, Pessah IN, Wulff H
Ann Clin Transl Neurol. 2022; 9(5):600-609. PMC9082378

Diisopropylfluorophosphate (DFP) volatizes and cross-contaminates wells in a common 96-well plate format used in zebrafish larvae toxicology studies.
Mundy PC, Mendieta R, Lein PJ.
J Pharmacol Toxicol Methods. 2022 May-Jun 2022;115:107173. PMCID: PMC9356770

A fast and selective approach for profiling vicinal diols using liquid chromatography-post column derivatization-double precursor ion scanning mass spectrometry
Wan D, Morisseau C, Hammock BD, Yang J
Molecules. 2022;27:283. PMCID: PMC8747065

Improved ELISA for linoleate-derived diols in human plasma utilizing a polyHRP-based secondary tracer
Singh N, Li D, McReynolds CB, Morisseau C, Hammock BD
Analytical Methods. 2022;14:1810-19. PMCID: PMC9116231 


Intramuscular dose of midazolam in rats producing exposure equivalent to recommended dose of Seizalam® (midazolam injection) in humans
Dhir A, Wu C-Y, Rogawski MA
Program No. P077.05 2021 Neuroscience Meeting Planner, p. 413. Chicago, IL: Society for Neuroscience, 2021. Online.

Estimation of the intramuscular dose of midazolam in mice to achieve the therapeutic plasma concentration effective for the treatment of status epilepticus in humans
Zolkowska D, Wu C-Y, Stone C, Rogawski MA
American Epilepsy Society Annual Meeting, Abstract 1.135, Translational Research, 2021 Dec 4. 

Sex-specific acute and chronic neurotoxicity of acute diisopropylfluorophosphate (DFP)-intoxication in juvenile Sprague-Dawley rats
González EA, Calsbeek JJ, Tsai Y-H, Tang M-Y, Andrew P, Vu J, Berg EL, Saito NH, Harvey DJ, Supasai S, Gurkoff GG, Silverman JL, Lein PJ
Current Research in Toxicology. 2021 Sep 14. 2:341-356. PMCID: PMC8484742

Persistent neuropathology and behavioral deficits in a mouse model of status epilepticus induced by acute intoxication with diisopropylfluorophosphate
Calsbeek JJ, González EA, Bruun DA, Guignet MA, Copping N, Dawson ME, Yu AJ, MacMahon JA, Saito NH, Harvey DJ, Silverman JL, Lein PJ.
Neurotoxicology. 2021 Sep 9:S0161-813X(21)00113-3. PMCID: PMC8595753

The efficacy of γ-aminobutyric acid type A receptor (GABA AR) subtype-selective positive allosteric modulators in blocking tetramethylenedisulfotetramine (TETS)-induced seizure-like behavior in larval zebrafish with minimal sedation
Mundy PC, Pressly B, Carty DR, Yaghoobi B, Wulff H, Lein PJ.
Toxicol Appl Pharmacol. 2021 Sep 1;426:115643. PMCID: PMC8514104

Strain differences in the extent of brain injury in mice after tetramethylenedisulfotetramine-induced status epilepticus
Calsbeek JJ, González EA, Boosalis CA, Zolkowska D, Bruun DA, Rowland DJ, Saito NH, Harvey DJ, Chaudhari AJ, Rogawski MA, Garbow JR, Lein PJ
NeuroToxicology. 2021 Aug 31; 87:43-50. PMCID: PMC8595842

Neuroinflammation as a therapeutic target for mitigating the long-term consequences of acute organophosphate intoxication
Andrew PM and Lein PJ
Front Pharmacol. 2021 May 12;12:674325. PMCID: PMC8153682

Mechanisms of organophosphate neurotoxicity
Tsai Y-H and Lein PJ
Current Opinion in Tox, 2021 April; 26:49-60. PMCID: PMC8302047 

Perampanel, a potent AMPA receptor antagonist, protects against tetramethylenedisulfotetramine-induced seizures and lethality in mice: comparison with diazepam
Zolkowska D, Dhir A, Rogawski
Arch Toxicol. 2021 Jul;95(7):2459-2468. PMCID: PMC8241714

Colocalization of fluorescent signals using deep learning with Manders overlapping coefficient
Dou Y, Tsai Y-H, Liu CC, Hobson BA, Lein PJ
Proc SPIE Int Soc Opt Eng. 2021 Feb;11596-115963C. PMCID: PMC8301216

Identification of the functional binding site for the convulsant tetramethylenedisulfotetramine in the pore of the α 2 β 3 γ 2 GABAA receptor
Pressly B, Lee RD, Barnych B, Hammock BD, Wulff H
Mol Pharmacol. 2021 Jan;99(1):78-91. PMCID: PMC7746976 

Structure-activity relationship of neuroactive steroids, midazolam, and perampanel towards mitigating tetramine-triggered activity in murine hippocampal neuronal networks
Antrobus S, Pressly B, Nik AM, Wulff H, Pessah IN
Toxicol Sci. 2021 Apr 12;180(2):325-341. PMCID: PMC8599726

Intranasal allopregnanolone confers rapid seizure protection: Evidence for direct nose-to-brain delivery
Zolkowska D, Wu CY, Rogawski MA
Neurotherapeutics. 2021 Jan;18(1):544-555. PMCID: PMC8116426

Letter to Editor: Requiring post-hoc power of 80% amounts to an unstated lowering of the type-1 error rate
Tancredi DJ, Harvey DJ, Smiley-Jewell S, Nguyen DV
Neurotoxicology. 2021 Jan;82:99. PMID: 33232746 

Plasma linoleate diols are potential biomarkers for severe COVID-19 infections
McReynolds CB, Cortes-Puch I, Ravindran R, Khan IH, Hammock BG, Shih P-aB, Hammock BD, Yang J
Frontiers in Physiology. 2021;12:663869. PMCID: PMC8047414

TPPU treatment of burned mice dampens inflammation and generation of bioactive DHET which impairs neutrophil function
Bergmann CB, Hammock BD, Wan D, Gogolla F, Goetzman H, Caldwell CC, Supp DM
Scientific Reports. 2021;11:16555. PMCID: PMC8368302

Species differences in metabolism of soluble epoxide hydrolase inhibitor, EC1728, highlight the importance of clinically relevant screening mechanisms in drug development
McReynolds CB, Yang J, Guedes A, Morisseau C, Garcia R, Knych H, Tearny C, Hwang SH, Wagner K, Hammock BD
Molecules. 2021;26:5034. PMCID: PMC8399023

Movement to the clinic of soluble epoxide hydrolase inhibitor EC5026 as an analgesic for neuropathic pain and for use as a nonaddictive opioid alternative
Hammock BD, McReynolds CB, Wagner K, Buckpitt A, Cortes-Puch I, Croston G, Lee KS, Yang J, Schmidt WK, Hwang SH
J Med Chem. 2021;64:1856-72. PMCID: PMC7917437

-- 2020 --

New capsaicin analogs as molecular rulers to define the permissive conformation of the mouse TRPV1 ligand-binding pocket
Vu S, Singh V, Wulff H, Yarov-Yarovoy V, Zheng J
eLife 2020 Nov 9;e62039. PMCID: PMC7671684

Acute administration of diazepam or midazolam minimally alters long-term neuropathological effects in the rat brain following acute intoxication with diisopropylfluorophosphate
Supasai S, González EA, Rowland DJ, Hobson B, Bruun DA, Guignet MA, Soares S, Singh V, Wulff H, Saito N, Harvey DJ, Lein PJ
Eur J Pharmacol. 2020 Nov 5;886:173538. PMCID: PMC7572820

Allopregnanolone and perampanel as adjuncts to midazolam for treating diisopropylfluorophosphate-induced status epilepticus in rats
Dhir A, Bruun DA, Guignet M, Tsai Y-H, González E, Calsbeek J, Vu J, Saito N, Tancredi DJ, Harvey DJ, Lein PJ, Rogawski MA
Ann NY Acad Sci 2020 Sep 11, 1-23. PMCID: PMC7756871

A national toxicology program systematic review of the evidence for long-term effects after acute exposure to sarin nerve agent
Jett DA, Sibrizzi CA, Blain RB, Hartman PA, Lein PJ, Taylor KW, Rooney AA
Critical Reviews in Toxicology 2020 Aug 5;1-17. PMCID: PMC8011809

Soluble epoxide hydrolase regulation of lipid mediators limits pain
Wagner KM, Gomes A, McReynolds CB, Hammock BD.
Neurotherapeutics. 2020 Jul;17(3):900-916. PMCID: PMC7609775

The chemical convulsant diisopropylfluorophosphate (DFP) causes persistent neuropathology in adult male rats independent of seizure activity
González EA, Rindy AC, Guignet MA, Calsbeek JJ, Bruun DA, Dhir A, Andrew P, Saito N, Rowland DJ, Harvey DJ, Rogawski MA, Lein PJ .
Archives In Toxicolology 2020 June;94:2149-2162. PMCID: PMC7305973

Comparison of the toxicokinetics of the convulsants picrotoxinin and tetramethylenedisulfotetramine (TETS) in mice
Pressly B. Vasylieva N. Barnych B, Singh V. Bruun DA, Hwang SH. Chen YJ, Johnnides S, Lein PJ. Yang J, Hammock BD, Wulff H
Archives in Toxicology 2020 June:9494(6):1995-2007PMCID: PMC7303059

Soluble epoxide hydrolase inhibitor mediated analgesia lacks tolerance in rat models
Wagner KM, Atone J, Hammock BD
Brain research. 2020 February 1;1728:146573. PMCID: PMC6996511

Pharmacology of small- and intermediate-conductance calcium-activated potassium channels
Brown BM, Shim H, Christophersen P, Wulff H
Annu Rev Pharmacol Toxicol. 2020;60:219-240. PMID 31337271

National Institutes of Neurological Disorders and Stroke (NINDS)/American Epilepsy Society (AES) epilepsy research benchmark stewards. Epilepsy benchmarks area III: Improved treatment options for controlling seizures and epilepsy-related conditions without side effects
Traynelis SF, Dlugos D, Henshall D, Mefford HC, Rogawski MA, Staley KJ, Dacks  PA, Whittemore V, Poduri A
Epilepsy Curr. 2020 Jan-Feb;20(1_suppl):23S-30S. PMCID: PMC7031805.

Susceptibility of larval zebrafish to the seizurogenic activity of GABA type A receptor antagonists
Bandara SB, Carty DR, Singh V, Harvey DJ, Vasylieva N, Pressly B, Wulff H, Lein PJ
Neurotoxicology. 2020 Jan;76:220-234. PMCID: PMC6957304

Persistent behavioral deficits, neuroinflammation and oxidative stress in a rat model of acute organophosphate intoxication
Guignet M, Dhakal K, Flannery BM, Hobson BA, Zolkowska D, Dhir A, Bruun DA, Li S, Harvey DJ, Silverman JL, Rogawski MA, Lein PJ
Neurobiol Dis. 2020 Jan;133:104431. PMCID: PMC6754818

-- 2019 --

A permethrin metabolite is associated with adaptive immune responses in Gulf War Illness
Joshi U, Pearson A, Evans JE, Langlois H. Saltiel N, Ojo J, Klimas N, Sullivan K, Keegan AP, Oberlin S, Darcey T, Cseresznye A, Raya B. Paris D, Hammock B, Vasylieva N, Hongsibsong S, Stern LJ, Crawford F, Mullan M, Abdullah L
Brain, Behavior, and Immunity 2019:81:545-559. PMID 31325531; PMCID: PMC7155744

Lipidomes of brain from rats acutely intoxicated with diisopropylfluorophosphate identifies potential therapeutic targets
Yang J, Bruun DA, Wang C, Wan D, McReynolds CB, Phu K, Inceoglu B, Lein PJ, Hammock BD
Toxicol Appl Pharmacol. 2019 Nov 1;382:114749. PMID: 31521729; PMCID: PMC6957308

Acute peripheral immune activation alters cytokine expression and glial activation in the early postnatal rat brain
Bruce M, Streifel KM, Boosalis CA, Heuer L, González EA, Li S, Harvey DJ, Lein PJ, Van de Water J
J Neuroinflammation. 2019 Oct 31;16(1):200. PMID: 31672161; PubMed Central PMCID: PMC6822372.

Pretreatment with pyridostigmine bromide has no effect on seizure behavior or 24 hour survival in the rat model of acute diisopropylfluorophosphate intoxication
Bruun DA, Guignet M, Harvey DJ, Lein PJ
Neurotoxicology. 2019 Jul;73:81-84 PMID: 30853371; PMCID: PMC66344995

The use of percent change in RR interval for data exclusion in analyzing 24 hour time domain heart rate variability in rats
Karey E, Pan S, Morris A, Bruun DA, Lein PJ, Chen CY
Front. Physiol., 2019 Jun;10:693. PMID: 31244671; PMCID: PMC6562196

TSPO PET using [18F]PBR111 reveals persistent neuroinflammation following acute diisopropylfluorophosphate intoxication in the rat
Hobson BA, Rowland DJ, Siso S, Guignet MA, Harmany ZT, Bandara S, Saito N, Harvey DJ, Bruun DA, Garbow JR, Chaudhari AF, Lein PJ 
Toxicol Sci. 2019: 170(2):330-344. PMC6657665; PMCID: PMC6657665

Antibodies and venom peptides: new modalities for ion channels
Wulff H, Christophersen P, Colussi P, Chandy KG, Yarov-Yarovoy V
Nat Rev Drug Discov. 2019 May;18(5):339-357. PMID: 30728472; PMCID: PMC6499689

Guignet M, Lein PJ
In: Advances in Neurotoxicology: Role of Inflammation in Environmental Neurotoxicity, volume 3 (Edited by M Aschner, LG Costa), Academic Press, Cambridge, MA, pp. 35-79. 2019

1-Trifluoromethoxyphenyl-3-(1-propionylpiperidin-4-yl) urea, a selective and potent dual inhibitor of soluble epoxide hydrolase and p38 kinase intervenes in Alzheimer's signaling in human nerve cells
Liang Z, Zhang B, Xu M, Morisseau C, Hwang SH, Hammock BD, Li QX
ACS Chem Neurosci. 2019;10:4018-4030. PMC 7028313; PMCID: PMC7028313

The pharmacokinetics of ketamine following intramuscular injection to F344 rats
Moeller B, Espelien B, Weber W, Kuehl P, Doyle-Eisele M, Garner CE, McDonald JD, Garcia E, Raulli R, Laney J
Drug Test Anal.2019 Jan;11(1):68-76. doi: 10.1002/dta.2468. PMID: 30027605; PMCID: .PMC6538562

Soluble epoxide hydrolase inhibitor, APAU, protects dopaminergic neurons against rotenone induced neurotoxicity: Implications for Parkinson's disease
Lakkappa N, Krishnamurthy PT, M D P, Hammock BD, Hwang SH
Neurotoxicology. 2019 January;70:135-145. PMID: 30472438; PMCID: PMC6873230

The trials and tribulations of structure assisted design of KCa channel activators
Shim H, Brown BM, Singh L, Singh V, Fettinger JC, Yarov-Yarovoy V, Wulff H
Front Pharmacol. 2019;10:972. PMC6764326; PMCID: PMC6764326

In vitro and in vivo metabolism of a potent inhibitor of soluble epoxide hydrolase, 1-(1-Propionylpiperidin-4-yl)-3-(4-(trifluoromethoxy)phenyl)urea
Wan D, Yang J, McReynolds CB, Barnych B, Wagner KM, Morisseau C, Hwang SH, Sun J, Blöcher R, Hammock BD 
Front. Pharmacol. 2019;10:464. PMC6520522; PMCID: PMC6520522

Anti-inflammatory treatment with a soluble epoxide hydrolase inhibitor attenuates seizures and epilepsy-associated depression in the LiCl-pilocarpine post-status epilepticus rat model
Shen Y, Peng W. Chen Q. Hammock BD, Liu J, Li D, Yang J, Ding J, Wang X
Brain, Behavior, and Immunity 2019;81:535-544. PMC6873816; PMCID: PMC6873816

-- 2018 --

A magnetic resonance imaging study of early brain injury in a rat model of acute DFP intoxication
Hobson BA, Rowland DJ, Supasai S, Harvey DJ, Lein PJ, Garbow JR
Neurotoxicology. 2018 May;66:170-178. PMID: 29183789; PMCID: PMC5940565

Intramuscular allopregnanolone and ganaxolone in a mouse model of treatment-resistant status epilepticus
Zolkowska D, Wu CY, Rogawski MA.
Epilepsia. 2018 October;59 Suppl 2:220-227. PMID: 29453777; PMCID: PMC6910080

Bifenthrin causes transcriptomic alterations in mTOR and ryanodine receptor-dependent signaling and delayed hyperactivity in developing zebrafish (Danio rerio)
Frank DF, Miller GW, Harvey DJ, Brander SM, Geist J, Connon RE, Lein PJ
Aquat Toxicol. 2018 Jul;200:50-61. PMID: 29727771

A Microfluidic Platform to Study Astrocyte Adhesion on Nanoporous Gold Thin Films
Hampe AE, Li Z, Sethi S, Lein PJ, Seker E
Nanomaterials (Basel, Switzerland). 2018 June 21;8(7).
PMID: 29933551; PMCID: PMC6070884.

Determination of minimal steady-state plasma level of diazepam causing seizure threshold elevation in rats
Dhir A, Rogawski MA
Epilepsia.2018 May;59(5):935-944. doi: 10.1111/epi.14069. Epub 2018 Apr 6. PMID: 29682729; PMCID: PMC5934328

Soluble epoxide hydrolase inhibition decreases reperfusion injury after focal cerebral ischemia
Tu R, Armstrong J, Lee KSS, Hammock BD, Sapirstein A, Koehler RC
Scientific Reports. 2018 March 27;8(1):5279. PMID: 29588470; PMCID: PMC5869703

COX-2/sEH Dual Inhibitor PTUPB Potentiates the Antitumor Efficacy of Cisplatin
Wang F, Zhang H, Ma AH, Yu W, Zimmermann M, Yang J, Hwang SH, Zhu D, Lin TY, Malfatti M, Turteltaub KW, Henderson PT, Airhart S, Hammock BD, Yuan J, de Vere White RW, Pan CX
Molecular cancer therapeutics. 2018 February;17(2):474-483. PMID: 29284644; PMCID: PMC5824635.

GABAA receptor subtype selectivity of the proconvulsant rodenticide TETS
Pressly B, Nguyen HM, Wulff H
Arch Toxicol. 2018 Feb;92(2):833-844. doi: 10.1007/s00204-017-2089-4. Epub 2017 Oct 16. PMID: 29038840

-- 2017 --

Structural Determinants for the Selectivity of the Positive KCa3.1 Gating Modulator 5-Methylnaphtho[2,1-d]oxazol-2-amine (SKA-121)
Brown BM, Shim H, Zhang M, Yarov-Yarovoy V, Wulff H
Mol Pharmacol. 2017 Oct;92(4):469-480. PMID: 28760780

Rapid Throughput Analysis of GABAAReceptor Subtype Modulators and Blockers Using DiSBAC1(3) Membrane Potential Red Dye
Nik AM, Pressly B, Singh V, Antrobus S, Hulsizer S, Rogawski MA, Wulff H, Pessah IN
Mol Pharmacol. 2017 Jul;92(1):88-99. PMID: 28428226

Editor's Highlight: Spatiotemporal Progression and Remission of Lesions in the Rat Brain Following Acute Intoxication With Diisopropylfluorophosphate
Sisó S, Hobson BA, Harvey DJ, Bruun DA, Rowland DJ, Garbow JR, Lein PJ
Toxicol Sci. 2017 Jun 1;157(2):330-341. PMID: 28329845

From the Cover: Magnetic Resonance Imaging Reveals Progressive Brain Injury in Rats Acutely Intoxicated With Diisopropylfluorophosphate
Hobson BA, Sisó S, Rowland DJ, Harvey DJ, Bruun DA, Garbow JR, Lein PJ
Toxicol Sci. 2017 Jun 1;157(2):342-353. PMID: 28329842

Hydroxy-fipronil is a new urinary biomarker of exposure to fipronil
Vasylieva N, Barnych B, Wan D, El-Sheikh EA, Nguyen HM, Wulff H, McMahen R, Strynar M, Gee SJ, Hammock BD
Environ Int. 2017 Jun;103:91-98. doi: 10.1016/j.envint.2017.03.012. Epub 2017 Mar 23. PMID: 28343720 

Soluble epoxide hydrolase as a therapeutic target for pain, inflammatory and neurodegenerative diseases
Wagner KM, McReynolds CB, Schmidt WK, Hammock BD
Pharmacol Ther. 2017 Jun 19. pii: S0163-7258(17)30154-7. PMID: 28642117 [

Soluble epoxide hydrolase inhibition alleviates neuropathy in Akita (Ins2 Akita) mice
Wagner K, Gilda J, Yang J, Wan D, Morisseau C, Gomes AV, Hammock BD
Behav Brain Res. 2017 May 30;326:69-76. PMID: 28259677

Synthesis of cyclooxygenase metabolites of 8,9-epoxyeicosatrienoic acid (EET): 11- and 15-hydroxy 8,9-EETs
Barnych B, Rand AA, Cajka T, Lee KSS, Hammock BD
Org Biomol Chem. 2017 May 23;15(20):4308-4313.  PMID: 28470279

Sensitive immunoassay for detection and quantification of the neurotoxin, tetramethylenedisulfotetramine
Vasylieva N, Barnych B, Rand A, Inceoglu B, Gee SJ, Hammock BD
Analytical chemistry. 2017 May 16;89(10):5612-5619. PMID: 28398746;  PMCID: PMC5920647.

Structural insights into the atomistic mechanisms of action of small molecule inhibitors targeting the KCa3.1 channel pore
Nguyen HM, Singh V, Pressly B, Jenkins DP, Wulff H, Yarov-Yarovoy V
Mol Pharmacol. 2017 Apr;91(4):392-402. PMID: 28126850 

Nanostructure introduces artifacts in quantitative immunofluorescence by influencing fluorophore intensity
Chapman CA, Zhu X, Chen H, Yanik AA, Lein PJ, Seker E
Sci Rep. 2017 Mar 27;7(1):427. PMID: 28348397 

Epoxy fatty acids mediate analgesia in murine diabetic neuropathy
Wagner K, Lee KS, Yang J, Hammock BD
Eur J Pain. 2017 Mar;21(3):456-465. PMID: 27634339 

Inhibition of soluble epoxide hydrolase augments astrocyte release of vascular endothelial growth factor and neuronal recovery after oxygen-glucose deprivation
Zhang Y, Hong G, Lee KS, Hammock BD, Gebremedhin D, Harder DR, Koehler RC, Sapirstein A
J Neurochem. 2017 Mar;140(5):814-825. PMID: 28002622 

Chemical synthesis and biological evaluation of ω-hydroxy polyunsaturated fatty acids
Hwang SH, Wagner K, Xu J, Yang J, Li X, Cao Z, Morisseau C, Lee KS, Hammock BD
Bioorg Med Chem Lett. 2017 Feb 1;27(3):620-625. PMID: 28025003

Influence of tetramethylenedisulfotetramine on synchronous calcium oscillations at distinct developmental stages of hippocampal neuronal cultures
Cao Z, Xu J, Hulsizer S, Cui Y, Dong Y, Pessah IN
Neurotoxicology. 2017 Jan;58:11-22. PMID: 27984050 

Nanoporous gold biointerfaces: modifying nanostructure to control neural cell coverage and enhance electrophysiological recording performance
Chapman CAR, Wang L, Chen H, Garrison J, Lein PJ, Seker E
Adv Funct Mater. 2017 Jan 19;27(3). pii: 1604631. PMID: 28626362 

Probing the orientation of inhibitor and epoxy-eicosatrienoic acid binding in the active site of soluble epoxide hydrolase
Lee KS, Henriksen NM, Ng CJ, Yang J, Jia W, Morisseau C, Andaya A, Gilson MK, Hammock BD
Arch Biochem Biophys. 2017 Jan 1;613:1-11. PMID: 27983948 

-- 2016 --

Persistent neuroinflammation and cognitive impairment in a rat model of acute diisopropylfluorophosphate intoxication
Flannery BM, Bruun DA, Rowland DJ, Banks CN, Austin AT, Kukis DL, Li Y, Ford BD, Tancredi DJ, Silverman JL, Cherry SR, Lein PJ
J Neuroinflammation. 2016 Oct 12;13(1):267. PMID: 27733171 

VHH antibodies: emerging reagents for the analysis of environmental chemicals
Bever CS, Dong JX, Vasylieva N, Barnych B, Cui Y, Xu ZL, Hammock BD, Gee SJ
Anal Bioanal Chem. 2016 Sep;408(22):5985-6002. PMID: 27209591 

Mechanisms of reduced astrocyte surface coverage in cortical neuron-glia co-cultures on nanoporous gold surfaces
Chapman CA, Chen H, Stamou M, Lein PJ, Seker E
Cell Mol Bioeng. 2016 Sep;9(3):433-442. PMID: 27795742 

Models to identify treatments for the acute and persistent effects of seizure-inducing chemical threat agents
Pessah IN, Rogawski MA, Tancredi DJ, Wulff H, Zolkowska D, Bruun DA, Hammock BD, Lein PJ
Ann N Y Acad Sci. 2016 Aug;1378(1):124-136. PMID: 27467073

Inhibition of soluble epoxide hydrolase as a novel approach to high dose diazepam induced hypotension
Ulu A, Inceoglu B, Yang J, Singh V, Vito S, Wulff H, Hammock BD
J Clin Toxicol. 2016 Jun;6(3). pii: 1000300. PMID: 28255523

Novel omega-3 fatty acid epoxygenase metabolite reduces kidney fibrosis
Sharma A, Hye Khan MA, Levick SP, Lee KS, Hammock BD, Imig JD
Int J Mol Sci. 2016 May 18;17(5). pii: E751. PMID: 27213332 

Dietary linoleic acid-induced alterations in pro- and anti-nociceptive lipid autacoids: Implications for idiopathic pain syndromes?
Ramsden CE, Ringel A, Majchrzak-Hong SF, Yang J, Blanchard H, Zamora D, Loewke JD, Rapoport SI, Hibbeln JR, Davis JM, Hammock BD, Taha AY
Mol Pain. 2016 Mar 10;12. pii: 1744806916636386. PMID: 27030719 

Pro-atherogenic role of smooth muscle Nox4-based NADPH oxidase
Tong X, Khandelwal AR, Wu X, Xu Z, Yu W, Chen C, Zhao W, Yang J, Qin Z, Weisbrod RM, Seta F, Ago T, Lee KS, Hammock BD, Sadoshima J,, Cohen RA, Zeng C
J Mol Cell Cardiol. 2016 Mar;92:30-40. PMID: 26812119

-- 2015 --

Recent developments in ion channel pharmacology
Wulff H, Christophersen P
Channels (Austin). 2015;9(6):335. PMID: 26646476 

Contrasting actions of a convulsant barbiturate and its anticonvulsant enantiomer on the α1 β3 γ2L GABAA receptor account for their in vivo effects
Desai R, Savechenkov PY, Zolkowska D, Ge RL, Rogawski MA, Bruzik KS, Forman SA, Raines DE, Miller KW
J Physiol. 2015 Nov 15;593(22):4943-61. PMID: 26378885 

Combined treatment with diazepam and allopregnanolone reverses tetramethylenedisulfotetramine (TETS)-induced calcium dysregulation in cultured neurons and protects TETS-intoxicated mice against lethal seizures
Bruun DA, Cao Z, Inceoglu B, Vito ST, Austin AT, Hulsizer S, Hammock BD, Tancredi DJ, Rogawski MA, Pessah IN, Lein PJ
Neuropharmacology. 2015 Aug;95:332-42. PMID: 25882826

Development of an immunoassay for the detection of the phenylpyrazole insecticide fipronil
Vasylieva N, Ahn KC, Barnych B, Gee SJ, Hammock BD.
Environ Sci Technol. 2015 Aug 18;49(16):10038-47. PMID: 26196357 

Endoplasmic reticulum stress in the peripheral nervous system is a significant driver of neuropathic pain
Inceoglu B, Bettaieb A, Trindade da Silva CA, Lee KS, Haj FG, Hammock BD
Proc Natl Acad Sci U S A. 2015 Jul 21;112(29):9082-7. PMID: 26150506

The Alzheimer's Disease Neuroimaging Initiative phase 2: Increasing the length, breadth, and depth of our understanding
Beckett LA, Donohue MC, Wang C, Aisen P, Harvey DJ, Saito N; Alzheimer's Disease Neuroimaging Initiative.
Alzheimers Dement. 2015 Jul;11(7):823-31. PMID: 26194315 

Peripheral FAAH and soluble epoxide hydrolase inhibitors are synergistically antinociceptive
Sasso O, Wagner K, Morisseau C, Inceoglu B, Hammock BD, Piomelli D
Pharmacol Res. 2015 Jul;97:7-15. PMID: 25882247 

Pharmacological gating modulation of small- and intermediate-conductance Ca(2+)-activated K(+) channels (KCa2.x and KCa3.1)
Christophersen P, Wulff H
Channels (Austin). 2015;9(6):336-43. PMID: 26217968

High salt intake increases blood pressure in normal rats: putative role of 20-HETE and no evidence on changes in renal vascular reactivity
Walkowska A, Kuczeriszka M, Sadowski J, Olszyñski KH, Dobrowolski L, Červenka L, Hammock BD, Kompanowska-Jezierska E
Kidney Blood Press Res. 2015;40(3):323-34. PMID: 26067851 

Rapid throughput analysis demonstrates that chemicals with distinct seizurogenic mechanisms differentially alter Ca2+ dynamics in networks formed by hippocampal neurons in culture
Cao Z, Zou X, Cui Y, Hulsizer S, Lein PJ, Wulff H, Pessah IN
Mol Pharmacol. 2015 Apr;87(4):595-605. doi: 10.1124/mol.114.096701. Epub 2015 Jan 12.
PMID: 25583085 [PubMed - indexed for MEDLINE]

Nanoporous gold as a neural interface coating: effects of topography, surface chemistry, and feature size
Chapman CA, Chen H, Stamou M, Biener J, Biener MM, Lein PJ, Seker E
ACS Appl Mater Interfaces. 2015 Apr 8;7(13):7093-100. PMID: 25706691 

Neuregulin-1 inhibits neuroinflammatory responses in a rat model of organophosphate-nerve agent-induced delayed neuronal injury
Li Y, Lein PJ, Ford GD, Liu C, Stovall KC, White TE, Bruun DA, Tewolde T, Gates AS, Distel TJ, Surles-Zeigler MC, Ford BD
J Neuroinflammation. 2015 Apr 2;12:64. PMID: 25880399

Current understanding of the mechanism of action of the antiepileptic drug lacosamide
Rogawski MA, Tofighy A, White HS, Matagne A, Wolff C
Epilepsy Res. 2015 Feb;110:189-205. PMID: 25616473

Potent natural soluble epoxide hydrolase inhibitors from Pentadiplandra brazzeana baillon: synthesis, quantification, and measurement of biological activities in vitro and in vivo
Kitamura S, Morisseau C, Inceoglu B, Kamita SG, De Nicola GR, Nyegue M, Hammock BD
PLoS One. 2015 Feb 6;10(2):e0117438. PMID: 25659109

PPARδ signaling mediates the cytotoxicity of DHA in H9c2 cells
Samokhvalov V, Zlobine I, Jamieson KL, Jurasz P, Chen C, Lee KS, Hammock BD, Seubert JM
Toxicol Lett. 2015 Jan 5;232(1):10-20. PMID: 25300478

The riluzole derivative 2-amino-6-trifluoromethylthio-benzothiazole (SKA-19), a mixed KCa2 activator and NaV blocker, is a potent novel anticonvulsant
Coleman N, Nguyen HM, Cao Z, Brown BM, Jenkins DP, Zolkowska D, Chen YJ, Tanaka BS, Goldin AL, Rogawski MA, Pessah IN, Wulff H
Neurotherapeutics. 2015 Jan;12(1):234-49. PMID: 25256961

Behavioral assessment of NIH Swiss mice acutely intoxicated with tetramethylenedisulfotetramine
Flannery BM, Silverman JL, Bruun DA, Puhger KR, McCoy MR, Hammock BD, Crawley JN, Lein PJ
Neurotoxicol Teratol. 2015 Jan-Feb;47:36-45. PMID: 25446016

-- 2014 --

Factors influencing adverse skin responses in rats receiving repeated subcutaneous injections and potential impact on neurobehavior
Levoe SN, Flannery BM, Brignolo L, Imai DM, Koehne A, Austin AT, Bruun DA, Tancredi DJ, Lein PJ
Curr Neurobiol. 2014 Dec 1;5(1-2):1-10. PMID: 25705100 

Pediatric super-refractory status epilepticus treated with allopregnanolone
Broomall E, Natale JE, Grimason M, Goldstein J, Smith CM, Chang C, Kanes S, Rogawski MA, Wainwright MS
Ann Neurol. 2014 Dec;76(6):911-5. PMID: 25363147 

Post-exposure administration of diazepam combined with soluble epoxide hydrolase inhibition stops seizures and modulates neuroinflammation in a murine model of acute TETS intoxication
Vito ST, Austin AT, Banks CN, Inceoglu B, Bruun DA, Zolkowska D, Tancredi DJ, Rogawski MA, Hammock BD, Lein PJ
Toxicol Appl Pharmacol. 2014 Dec 1;281(2):185-94. PMID: 25448683 

The role of long chain fatty acids and their epoxide metabolites in nociceptive signaling
Wagner K, Vito S, Inceoglu B, Hammock BD
Prostaglandins Other Lipid Mediat. 2014 Oct;113-115:2-12. PMID: 25240260 

Anticonvulsant potencies of the enantiomers of the neurosteroids androsterone and etiocholanolone exceed those of the natural forms
Zolkowska D, Dhir A, Krishnan K, Covey DF, Rogawski MA
Psychopharmacology (Berl). 2014 Sep;231(17):3325-32. PMID: 24705905 

New positive Ca2+-activated K+ channel gating modulators with selectivity for KCa3.1
Coleman N, Brown BM, Oliván-Viguera A, Singh V, Olmstead MM, Valero MS, Köhler R, Wulff H
Mol Pharmacol. 2014 Sep;86(3):342-57. PMID: 24958817 

Perampanel inhibition of AMPA receptor currents in cultured hippocampal neurons
Chen CY, Matt L, Hell JW, Rogawski MA
PLoS One. 2014 Sep 17;9(9):e108021. PMID: 25229608 

Optimized inhibitors of soluble epoxide hydrolase improve in vitro target residence time and in vivo efficacy
Lee KS, Liu JY, Wagner KM, Pakhomova S, Dong H, Morisseau C, Fu SH, Yang J, Wang P, Ulu A, Mate CA, Nguyen LV, Hwang SH, Edin ML, Mara AA, Wulff H, Newcomer ME, Zeldin DC, Hammock BD
J Med Chem. 2014 Aug 28;57(16):7016-30. PMID: 25079952 

GABAA receptor target of tetramethylenedisulfotetramine
Zhao C, Hwang SH, Buchholz BA, Carpenter TS, Lightstone FC, Yang J, Hammock BD, Casida JE
Proc Natl Acad Sci U S A.2014 Jul 29;111(30):11223. Lightstone, Felice [corrected to Lightstone, Felice C]. PMID: 24912155 

Nanomolar bifenthrin alters synchronous Ca2+ oscillations and cortical neuron development independent of sodium channel activity
Cao Z, Cui Y, Nguyen HM, Jenkins DP, Wulff H, Pessah IN
Mol Pharmacol. 2014 Apr;85(4):630-9. PMID: 24482397 

The potential of antiseizure drugs and agents that act on novel molecular targets as antiepileptogenic treatments
Kaminski RM, Rogawski MA, Klitgaard H
Neurotherapeutics. 2014 Apr;11(2):385-400. PMID: 24671870 

Role of GluK1 kainate receptors in seizures, epileptic discharges, and epileptogenesis
Fritsch B, Reis J, Gasior M, Kaminski RM, Rogawski MA
J Neurosci. 2014 Apr 23;34(17):5765-75. PMID: 24760837 

Inhibition of soluble epoxide hydrolase is renoprotective in 5/6 nephrectomized Ren-2 transgenic hypertensive rats
Kujal P, Čertíková Chábová V, Škaroupková P, Husková Z, Vernerová Z, Kramer HJ, Walkowska A, Kompanowska-Jezierska E, Sadowski J, Kitada K, Nishiyama A, Hwang SH, Hammock BD, Imig JD, Červenka L
Clin Exp Pharmacol Physiol. 2014 Mar;41(3):227-37. PMID: 24471737 [

-- 2013 --

Soluble epoxide hydrolase inhibitor trans-4-[4-(3-adamantan-1-yl-ureido)-cyclohexyloxy]-benzoic acid is neuroprotective in rat model of ischemic stroke
Shaik JS, Ahmad M, Li W, Rose ME, Foley LM, Hitchens TK, Graham SH, Hwang SH, Hammock BD, Poloyac SM
Am J Physiol Heart Circ Physiol. 2013 Dec 1;305(11):H1605-13. PMID: 24043255

Epoxy fatty acids and inhibition of the soluble epoxide hydrolase selectively modulate GABA mediated neurotransmission to delay onset of seizures
Inceoglu B, Zolkowska D, Yoo HJ, Wagner KM, Yang J, Hackett E, Hwang SH, Lee KS, Rogawski MA, Morisseau C, Hammock BD
PLoS One. 2013 Dec 11;8(12):e80922. PMID: 24349022

Development of a QPatch automated electrophysiology assay for identifying KCa3.1 inhibitors and activators
Jenkins DP, Yu W, Brown BM, Løjkner LD, Wulff H
Assay Drug Dev Technol. 2013 Nov-Dec;11(9-10):551-60. PMID: 24351043

Proconvulsant actions of intrahippocampal botulinum neurotoxin B in the rat
Bröer S, Zolkowska D, Gernert M, Rogawski MA
Neuroscience. 2013 Nov 12;252:253-61. PMID: 23906638

The therapeutic potential of small-conductance KCa2 channels in neurodegenerative and psychiatric diseases
Lam J, Coleman N, Garing AL, Wulff H
Expert Opin Ther Targets. 2013 Oct;17(10):1203-20. PMID: 23883298

Long-lasting attenuation of amygdala-kindled seizures after convection-enhanced delivery of botulinum neurotoxins a and B into the amygdala in rats
Gasior M, Tang R, Rogawski MA
J Pharmacol Exp Ther. 2013 Sep;346(3):528-34. PMID: 23772062

Isolation of alpaca anti-idiotypic heavy-chain single-domain antibody for the aflatoxin immunoassay
Wang Y, Li P, Majkova Z, Bever CR, Kim HJ, Zhang Q, Dechant JE, Gee SJ, Hammock BD
Anal Chem. 2013 Sep 3;85(17):8298-303. PMID: 23965250

Neuroactive steroids for the treatment of status epilepticus
Rogawski MA, Loya CM, Reddy K, Zolkowska D, Lossin C
Epilepsia. 2013 Sep;54 Suppl 6:93-8. PMID: 24001085

Epilepsy therapy development: technical and methodologic issues in studies with animal models
Galanopoulou AS, Kokaia M, Loeb JA, Nehlig A, Pitkänen A, Rogawski MA, Staley KJ, Whittemore VH, Dudek FE
Epilepsia. 2013 Aug;54 Suppl 4:13-23. PMID: 23909850

Synthesis and biological evaluation of sorafenib- and regorafenib-like sEH inhibitors
Hwang SH, Wecksler AT, Zhang G, Morisseau C, Nguyen LV, Fu SH, Hammock BD
Bioorg Med Chem Lett. 2013 Jul 1;23(13):3732-7. PMID: 23726028

Therapeutic activity of inhibition of the soluble epoxide hydrolase in a mouse model of scrapie
Poli G, Corda E, Martino PA, Dall'ara P, Bareggi SR, Bondiolotti G, Iulini B, Mazza M, Casalone C, Hwang SH, Hammock BD, Inceoglu B
Life Sci. 2013 Jun 21;92(23):1145-50. PMID: 23651659 

Förster resonance energy transfer competitive displacement assay for human soluble epoxide hydrolase.
Lee KS, Morisseau C, Yang J, Wang P, Hwang SH, Hammock BD
Anal Biochem. 2013 Mar 15;434(2):259-68. PMID: 23219719

Phage-displayed peptide that mimics aflatoxins and its application in immunoassay
Wang Y, Wang H, Li P, Zhang Q, Kim HJ, Gee SJ, Hammock BD
J Agric Food Chem. 2013 Mar 13;61(10):2426-33. PMID: 23394544 

Rationally designed multitarget agents against inflammation and pain
Hwang SH, Wecksler AT, Wagner K, Hammock BD
Curr Med Chem. 2013;20(13):1783-99. Review. PMID: 23410172 

AMPA receptors as a molecular target in epilepsy therapy
Rogawski MA
Acta Neurol Scand Suppl. 2013;(197):9-18. PMID: 23480151 

Preclinical pharmacology of perampanel, a selective non-competitive AMPA receptor antagonist
Rogawski MA, Hanada T
Acta Neurol Scand Suppl. 2013;(197):19-24. PMID: 23480152 

-- 2012 --

Tetramethylenedisulfotetramine alters Ca²⁺ dynamics in cultured hippocampal neurons: mitigation by NMDA receptor blockade and GABA(A) receptor-positive modulation
Cao Z, Hammock BD, McCoy M, Rogawski MA, Lein PJ, Pessah IN
Toxicol Sci. 2012 Dec;130(2):362-72. PMID: 22889812 

Importance of membrane selection in the development of immunochromatographic assays for low-molecular weight compounds
Lee JY, Kim YA, Kim MY, Lee YT, Hammock BD, Lee HS
Anal Chim Acta. 2012 Dec 13;757:69-74. PMID: 23206398 

Neuregulin-1 is neuroprotective in a rat model of organophosphate-induced delayed neuronal injury
Li Y, Lein PJ, Liu C, Bruun DA, Giulivi C, Ford GD, Tewolde T, Ross-Inta C, Ford BD
Toxicol Appl Pharmacol. 2012 Jul 15;262(2):194-204. PMID: 22583949

Spatiotemporal patterns of GFAP upregulation in rat brain following acute intoxication with diisopropylfluorophosphate (DFP)
Liu C, Li Y, Lein PJ, Ford BD
Curr Neurobiol. 2012 Jul;3(2):90-97.PMID: 24039349

Characterization of seizures induced by acute and repeated exposure to tetramethylenedisulfotetramine
Zolkowska D, Banks CN, Dhir A, Inceoglu B, Sanborn JR, McCoy MR, Bruun DA, Hammock BD, Lein PJ, Rogawski MA
J Pharmacol Exp Ther. 2012 May;341(2):435-46. PMID: 22328574